Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 128253..128778 Replicon plasmid pLD39-1-134kb
Accession NZ_CP047660
Organism Escherichia coli strain LD39-1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag GUU82_RS24540 Protein ID WP_001159868.1
Coordinates 128253..128558 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag GUU82_RS24545 Protein ID WP_000813634.1
Coordinates 128560..128778 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU82_RS24525 124215..125381 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
GUU82_RS24530 125969..126724 - 756 WP_000852146.1 replication initiation protein RepE -
GUU82_RS24535 127446..128252 - 807 WP_000016982.1 site-specific integrase -
GUU82_RS24540 128253..128558 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
GUU82_RS24545 128560..128778 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GUU82_RS24550 129492..132113 + 2622 WP_000283688.1 histidinol-phosphatase -
GUU82_RS24555 132312..132542 + 231 WP_001261274.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GUU82_RS24560 132631..132906 + 276 WP_001403920.1 type II toxin-antitoxin system VapC family toxin -
GUU82_RS24565 132911..133128 - 218 Protein_129 transposase -
GUU82_RS24570 133129..133659 - 531 WP_000348883.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..134831 134831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T147103 WP_001159868.1 NZ_CP047660:c128558-128253 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T147103 NZ_CP062865:c1066066-1065848 [Escherichia coli O39:H21]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT147103 WP_000813634.1 NZ_CP047660:c128778-128560 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT147103 NZ_CP062865:c1066466-1066092 [Escherichia coli O39:H21]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References