Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 71044..71603 Replicon plasmid pLD39-1-134kb
Accession NZ_CP047660
Organism Escherichia coli strain LD39-1

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag GUU82_RS24200 Protein ID WP_089629733.1
Coordinates 71044..71319 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag GUU82_RS24205 Protein ID WP_089629734.1
Coordinates 71319..71603 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU82_RS24180 67178..67462 - 285 WP_160183294.1 hypothetical protein -
GUU82_RS24185 68742..69248 + 507 WP_045173867.1 thiol peroxidase -
GUU82_RS24805 69308..69511 - 204 Protein_56 IS3 family transposase -
GUU82_RS24190 69821..70141 + 321 Protein_57 transposase family protein -
GUU82_RS24195 70168..70920 - 753 Protein_58 DUF4158 domain-containing protein -
GUU82_RS24200 71044..71319 - 276 WP_089629733.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GUU82_RS24205 71319..71603 - 285 WP_089629734.1 ribbon-helix-helix domain-containing protein Antitoxin
GUU82_RS24210 72511..73367 - 857 Protein_61 incFII family plasmid replication initiator RepA -
GUU82_RS24215 73360..73434 - 75 WP_061357229.1 RepA leader peptide Tap -
GUU82_RS24220 73666..73923 - 258 WP_000083845.1 replication regulatory protein RepA -
GUU82_RS24225 74207..74413 - 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
- 74400..74461 + 62 NuclAT_1 - -
- 74400..74461 + 62 NuclAT_1 - -
- 74400..74461 + 62 NuclAT_1 - -
- 74400..74461 + 62 NuclAT_1 - -
GUU82_RS24230 74803..75015 - 213 WP_032153777.1 hypothetical protein -
GUU82_RS24235 75146..75706 - 561 WP_021563950.1 fertility inhibition protein FinO -
GUU82_RS24240 75761..76501 - 741 WP_089590496.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..134831 134831
- flank IS/Tn - - 65979..66950 971


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10742.41 Da        Isoelectric Point: 9.5302

>T147100 WP_089629733.1 NZ_CP047660:c71319-71044 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFETWEVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T147100 NZ_CP062865:c569754-569545 [Escherichia coli O39:H21]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTAGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10464.90 Da        Isoelectric Point: 6.4632

>AT147100 WP_089629734.1 NZ_CP047660:c71603-71319 [Escherichia coli]
MRMKNNSAQATKVITAHVSLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR

Download         Length: 285 bp

>AT147100 NZ_CP062865:569746-569803 [Escherichia coli O39:H21]
GTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References