Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 87757..89380 Replicon plasmid pLD39-1-MCR1
Accession NZ_CP047659
Organism Escherichia coli strain LD39-1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag GUU82_RS23015 Protein ID WP_000088046.1
Coordinates 88055..89380 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag GUU82_RS23010 Protein ID WP_024136327.1
Coordinates 87757..88050 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU82_RS22985 83629..84870 + 1242 WP_001053338.1 VWA domain-containing protein -
GUU82_RS22990 85433..85714 - 282 WP_000077926.1 hypothetical protein -
GUU82_RS22995 85764..85955 - 192 WP_000398480.1 hypothetical protein -
GUU82_RS23000 86047..86388 - 342 WP_001151575.1 hypothetical protein -
GUU82_RS23005 86761..87153 + 393 WP_000341066.1 hypothetical protein -
GUU82_RS23010 87757..88050 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
GUU82_RS23015 88055..89380 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
GUU82_RS23020 89441..89647 + 207 WP_000134171.1 hypothetical protein -
GUU82_RS23025 89749..90159 + 411 WP_000985911.1 hypothetical protein -
GUU82_RS23030 90172..90987 + 816 WP_146881499.1 HNH endonuclease -
GUU82_RS23035 91241..91666 + 426 WP_001043843.1 hypothetical protein -
GUU82_RS23040 92215..92523 + 309 WP_001224687.1 hypothetical protein -
GUU82_RS23045 92539..93396 + 858 WP_000784387.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / mph(A) / blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / sul1 - 1..251000 251000


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T147099 WP_000088046.1 NZ_CP047659:88055-89380 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T147099 NZ_CP062865:382708-383058 [Escherichia coli O39:H21]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT147099 WP_024136327.1 NZ_CP047659:87757-88050 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT147099 NZ_CP062865:382463-382714 [Escherichia coli O39:H21]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References