Detailed information of TA system TA147093

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3731512..3732191
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: GUU82_RS18115
• Protein ID: WP_000854680.1
• Coordinates: 3731850..3732191 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: GUU82_RS18110
• Protein ID: WP_000070396.1
• Coordinates: 3731512..3731829 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS18065	3726950..3727771	+	822	WP_023565123.1	DUF945 domain-containing protein	-
GUU82_RS18070	3727988..3728689	+	702	WP_160183271.1	WYL domain-containing protein	-
GUU82_RS18075	3728730..3728966	+	237	WP_160183272.1	hypothetical protein	-
GUU82_RS18080	3728966..3729409	+	444	WP_000649865.1	hypothetical protein	-
GUU82_RS18085	3729432..3729899	+	468	WP_001385283.1	hypothetical protein	-
GUU82_RS18090	3729976..3730215	+	240	Protein_3533	DUF905 domain-containing protein	-
GUU82_RS18095	3730313..3730771	+	459	WP_000211838.1	antirestriction protein	-
GUU82_RS18100	3730787..3731263	+	477	WP_000811693.1	RadC family protein	-
GUU82_RS18105	3731272..3731493	+	222	WP_000691994.1	DUF987 domain-containing protein	-
GUU82_RS18110	3731512..3731829	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GUU82_RS18115	3731850..3732191	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GUU82_RS18125	3732763..3734016	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
GUU82_RS18130	3734028..3735131	-	1104	WP_160183273.1	glutamate 5-kinase	-
GUU82_RS18135	3735419..3736474	+	1056	WP_000749875.1	phosphoporin PhoE	-
GUU82_RS18140	3736513..3736914	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T147093 WP_000854680.1 NZ_CP047658:3731850-3732191 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T147093 NZ_CP062863:c3678870-3678472 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT147093 WP_000070396.1 NZ_CP047658:3731512-3731829 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT147093 NZ_CP062863:c3679166-3678873 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7