Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2524233..2524871 Replicon chromosome
Accession NZ_CP047658
Organism Escherichia coli strain LD39-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GUU82_RS12255 Protein ID WP_000813794.1
Coordinates 2524695..2524871 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GUU82_RS12250 Protein ID WP_001270286.1
Coordinates 2524233..2524649 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU82_RS12230 2519385..2520326 - 942 WP_001251313.1 ABC transporter permease -
GUU82_RS12235 2520327..2521340 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
GUU82_RS12240 2521358..2522503 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GUU82_RS12245 2522748..2524154 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GUU82_RS12250 2524233..2524649 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GUU82_RS12255 2524695..2524871 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GUU82_RS12260 2525093..2525323 + 231 WP_000494244.1 YncJ family protein -
GUU82_RS12265 2525415..2527376 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GUU82_RS12270 2527449..2527985 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GUU82_RS12275 2528038..2529252 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T147090 WP_000813794.1 NZ_CP047658:c2524871-2524695 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T147090 NZ_CP062863:2643059-2643166 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT147090 WP_001270286.1 NZ_CP047658:c2524649-2524233 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT147090 NZ_CP062863:c2643012-2642946 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References