Detailed information of TA system TA147088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2326828..2327354
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: GUU82_RS11220
• Protein ID: WP_000323025.1
• Coordinates: 2327067..2327354 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: GUU82_RS11215
• Protein ID: WP_000534858.1
• Coordinates: 2326828..2327067 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS11185	2321925..2322167	+	243	Protein_2185	DUF977 family protein	-
GUU82_RS11190	2322164..2322520	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
GUU82_RS24680	2323131..2323418	+	288	Protein_2187	hypothetical protein	-
GUU82_RS11200	2324188..2325501	+	1314	WP_000589005.1	ISNCY family transposase	-
GUU82_RS11205	2325963..2326295	-	333	WP_001301033.1	protein FlxA	-
GUU82_RS11210	2326498..2326803	-	306	WP_001326990.1	hypothetical protein	-
GUU82_RS11215	2326828..2327067	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
GUU82_RS11220	2327067..2327354	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
GUU82_RS11225	2327426..2327581	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
GUU82_RS11230	2327798..2328049	+	252	WP_000980994.1	hypothetical protein	-
GUU82_RS11235	2328116..2328394	+	279	WP_012304870.1	hypothetical protein	-
GUU82_RS11240	2328396..2329445	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
GUU82_RS11245	2329459..2330211	+	753	WP_001047135.1	antitermination protein	-
GUU82_RS11250	2330489..2330578	-	90	WP_120795389.1	hypothetical protein	-
GUU82_RS11255	2330633..2330845	-	213	WP_000087756.1	cold shock-like protein CspF	-
GUU82_RS11260	2331146..2331361	+	216	WP_000066484.1	cold shock-like protein CspB	-
GUU82_RS11265	2332115..2332330	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2314061..2344441	30380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T147088 WP_000323025.1 NZ_CP047658:2327067-2327354 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T147088 NZ_CP062863:2271796-2272083 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT147088 WP_000534858.1 NZ_CP047658:2326828-2327067 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT147088 NZ_CP062863:2271557-2271796 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8