Detailed information of TA system TA147079

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1884971..1885803
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GUU82_RS08950
• Protein ID: WP_112022308.1
• Coordinates: 1884971..1885345 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: GUU82_RS08955
• Protein ID: WP_001295723.1
• Coordinates: 1885435..1885803 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS08915	1880430..1880759	+	330	WP_000450409.1	DUF496 family protein	-
GUU82_RS08920	1880860..1881042	-	183	WP_001667686.1	ethanolamine utilization - propanediol utilization family protein	-
GUU82_RS08930	1881316..1882098	-	783	WP_112022309.1	IS21-like element IS100kyp family helper ATPase IstB	-
GUU82_RS08935	1882095..1883117	-	1023	WP_000255946.1	IS21-like element IS100 family transposase	-
GUU82_RS24670	1883455..1883844	-	390	WP_077249349.1	transposase	-
GUU82_RS24675	1884642..1884755	-	114	WP_001161660.1	DUF957 domain-containing protein	-
GUU82_RS08945	1884768..1884974	-	207	WP_000976829.1	hypothetical protein	-
GUU82_RS08950	1884971..1885345	-	375	WP_112022308.1	TA system toxin CbtA family protein	Toxin
GUU82_RS08955	1885435..1885803	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GUU82_RS08960	1885966..1886187	-	222	WP_000692345.1	DUF987 domain-containing protein	-
GUU82_RS08965	1886250..1886726	-	477	WP_001186774.1	RadC family protein	-
GUU82_RS08970	1886742..1887215	-	474	WP_000855059.1	antirestriction protein	-
GUU82_RS08975	1887517..1888335	-	819	WP_016231181.1	DUF945 domain-containing protein	-
GUU82_RS08980	1888490..1888648	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	wza / gmd / wcaG / manB / wcaJ / galF / wbtL / gnd / ugd / gtrB	1775977..1939781	163804

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14112.18 Da
• Isoelectric Point: 7.1881

>T147079 WP_112022308.1 NZ_CP047658:c1885345-1884971 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLMNSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T147079 NZ_CP062863:662395-662709 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT147079 WP_001295723.1 NZ_CP047658:c1885803-1885435 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT147079 NZ_CP062863:662706-663122 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52