Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4165072..4165691 Replicon chromosome
Accession NZ_CP047649
Organism Klebsiella pneumoniae strain 158590

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GTK59_RS20410 Protein ID WP_002892050.1
Coordinates 4165473..4165691 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GTK59_RS20405 Protein ID WP_002892066.1
Coordinates 4165072..4165446 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK59_RS20395 4160224..4161417 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GTK59_RS20400 4161440..4164586 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
GTK59_RS20405 4165072..4165446 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GTK59_RS20410 4165473..4165691 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GTK59_RS20415 4165850..4166416 + 567 WP_002892030.1 maltose O-acetyltransferase -
GTK59_RS26940 4166388..4166528 - 141 WP_004147370.1 hypothetical protein -
GTK59_RS20420 4166549..4167019 + 471 WP_002892026.1 YlaC family protein -
GTK59_RS20425 4166994..4168445 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GTK59_RS20430 4168546..4169244 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
GTK59_RS20435 4169241..4169381 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GTK59_RS20440 4169381..4169644 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T147058 WP_002892050.1 NZ_CP047649:4165473-4165691 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T147058 NZ_CP062855:1382676-1383074 [Escherichia coli O20:H12]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT147058 WP_002892066.1 NZ_CP047649:4165072-4165446 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT147058 NZ_CP062855:1382449-1382676 [Escherichia coli O20:H12]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References