Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5337754..5338379 Replicon chromosome
Accession NZ_CP047648
Organism Klebsiella pneumoniae strain 156070

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GTK58_RS25940 Protein ID WP_002882817.1
Coordinates 5337754..5338137 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GTK58_RS25945 Protein ID WP_004150355.1
Coordinates 5338137..5338379 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK58_RS25925 5335120..5336022 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GTK58_RS25930 5336019..5336654 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GTK58_RS25935 5336651..5337580 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GTK58_RS25940 5337754..5338137 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GTK58_RS25945 5338137..5338379 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GTK58_RS25950 5338584..5339501 + 918 WP_002882812.1 alpha/beta hydrolase -
GTK58_RS25955 5339515..5340456 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GTK58_RS25960 5340501..5340938 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GTK58_RS25965 5340935..5341795 - 861 WP_002882807.1 virulence factor BrkB family protein -
GTK58_RS25970 5341789..5342388 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T147044 WP_002882817.1 NZ_CP047648:c5338137-5337754 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T147044 NZ_CP062853:3650012-3650290 [Escherichia coli O9:H10]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT147044 WP_004150355.1 NZ_CP047648:c5338379-5338137 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT147044 NZ_CP062853:3649749-3650009 [Escherichia coli O9:H10]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References