Detailed information of TA system TA147022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2204951..2205480
• Replicon: chromosome
• Accession: NZ_CP047646
• Organism: Staphylococcus aureus strain 19/Msa0875

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GQ601_RS10485
• Protein ID: WP_000621175.1
• Coordinates: 2205118..2205480 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GQ601_RS10480
• Protein ID: WP_000948331.1
• Coordinates: 2204951..2205121 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ601_RS10450 (GQ601_10500)	2199987..2200547	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
GQ601_RS10455 (GQ601_10505)	2200756..2201235	+	480	WP_001287081.1	hypothetical protein	-
GQ601_RS10460 (GQ601_10510)	2201228..2202805	+	1578	WP_001294650.1	PH domain-containing protein	-
GQ601_RS10465 (GQ601_10515)	2202798..2203289	+	492	WP_001286980.1	PH domain-containing protein	-
GQ601_RS10470 (GQ601_10520)	2203293..2203652	+	360	WP_000581197.1	holo-ACP synthase	-
GQ601_RS10475 (GQ601_10525)	2203718..2204866	+	1149	WP_001281139.1	alanine racemase	-
GQ601_RS10480 (GQ601_10530)	2204951..2205121	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GQ601_RS10485 (GQ601_10535)	2205118..2205480	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GQ601_RS10490 (GQ601_10540)	2205830..2206831	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GQ601_RS10495 (GQ601_10545)	2206950..2207276	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GQ601_RS10500 (GQ601_10550)	2207278..2207757	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
GQ601_RS10505 (GQ601_10555)	2207732..2208502	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147022 WP_000621175.1 NZ_CP047646:2205118-2205480 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147022 NZ_CP062851:3031928-3032314 [Escherichia coli O9:H10]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147022 WP_000948331.1 NZ_CP047646:2204951-2205121 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147022 NZ_CP062851:3032304-3032633 [Escherichia coli O9:H10]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1