Detailed information of TA system TA147013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1308878..1309795
• Replicon: chromosome
• Accession: NZ_CP047644
• Organism: Bacillus sp. AM1(2019)

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GNE05_RS06870
• Protein ID: WP_007407256.1
• Coordinates: 1309049..1309795 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GNE05_RS06865
• Protein ID: WP_003154807.1
• Coordinates: 1308878..1309048 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNE05_RS06830	1304090..1305679	+	1590	WP_029973066.1	hypothetical protein	-
GNE05_RS06835	1305692..1306117	+	426	WP_029973065.1	hypothetical protein	-
GNE05_RS06840	1306122..1306319	+	198	WP_007610833.1	XkdX family protein	-
GNE05_RS06845	1306376..1307137	+	762	WP_160182604.1	phage portal protein	-
GNE05_RS06850	1307189..1307452	+	264	WP_022553473.1	hemolysin XhlA family protein	-
GNE05_RS06855	1307466..1307729	+	264	WP_057079949.1	phage holin	-
GNE05_RS06860	1307743..1308621	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GNE05_RS06865	1308878..1309048	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GNE05_RS06870	1309049..1309795	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GNE05_RS06875	1309900..1310898	-	999	WP_003154805.1	inorganic phosphate transporter	-
GNE05_RS06880	1310911..1311528	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GNE05_RS06885	1311814..1313130	-	1317	WP_007610842.1	amino acid permease	-
GNE05_RS06890	1313452..1314402	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1274438..1367877	93439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T147013 WP_007407256.1 NZ_CP047644:c1309795-1309049 [Bacillus sp. AM1(2019)]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T147013 NZ_CP062851:1759251-1759502 [Escherichia coli O9:H10]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT147013 WP_003154807.1 NZ_CP047644:c1309048-1308878 [Bacillus sp. AM1(2019)]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT147013 NZ_CP062851:1759499-1759753 [Escherichia coli O9:H10]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14