Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 536671..537308 Replicon chromosome
Accession NZ_CP047644
Organism Bacillus sp. AM1(2019)

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GNE05_RS02795 Protein ID WP_003156187.1
Coordinates 536958..537308 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GNE05_RS02790 Protein ID WP_003156188.1
Coordinates 536671..536952 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNE05_RS02770 533036..533635 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
GNE05_RS02775 533728..534093 + 366 WP_160182406.1 holo-ACP synthase -
GNE05_RS02780 534258..535265 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
GNE05_RS02785 535382..536551 + 1170 WP_160182407.1 alanine racemase -
GNE05_RS02790 536671..536952 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GNE05_RS02795 536958..537308 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GNE05_RS02800 537426..538247 + 822 WP_003156182.1 STAS domain-containing protein -
GNE05_RS02805 538252..538617 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
GNE05_RS02810 538620..539021 + 402 WP_003156178.1 anti-sigma regulatory factor -
GNE05_RS02815 539033..540040 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
GNE05_RS02820 540104..540433 + 330 WP_033575044.1 anti-sigma factor antagonist -
GNE05_RS02825 540430..540912 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GNE05_RS02830 540878..541666 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
GNE05_RS02835 541666..542268 + 603 WP_160182408.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T147012 WP_003156187.1 NZ_CP047644:536958-537308 [Bacillus sp. AM1(2019)]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T147012 NZ_CP062851:976659-976994 [Escherichia coli O9:H10]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT147012 WP_003156188.1 NZ_CP047644:536671-536952 [Bacillus sp. AM1(2019)]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT147012 NZ_CP062851:976411-976659 [Escherichia coli O9:H10]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References