Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 4692240..4693498 Replicon chromosome
Accession NZ_CP047643
Organism Pseudomonas aeruginosa CI27

Toxin (Protein)


Gene name - Uniprot ID A0A0H2Z7F3
Locus tag GTW26_RS21965 Protein ID WP_003140635.1
Coordinates 4692240..4692995 (-) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag GTW26_RS21970 Protein ID WP_003086329.1
Coordinates 4693130..4693498 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTW26_RS21955 (GTW26_21930) 4687411..4689954 + 2544 WP_003140632.1 acyl-homoserine-lactone acylase QuiP -
GTW26_RS21960 (GTW26_21935) 4690109..4691470 - 1362 WP_003081943.1 DNA recombination protein RmuC -
GTW26_RS21965 (GTW26_21940) 4692240..4692995 - 756 WP_003140635.1 RES family NAD+ phosphorylase Toxin
GTW26_RS21970 (GTW26_21945) 4693130..4693498 - 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
GTW26_RS21975 (GTW26_21950) 4693571..4694857 - 1287 WP_003086327.1 FAD-binding oxidoreductase -
GTW26_RS21980 (GTW26_21955) 4694913..4696406 - 1494 WP_016254122.1 aldehyde dehydrogenase family protein -
GTW26_RS21985 (GTW26_21960) 4696581..4697057 + 477 WP_003140638.1 hypothetical protein -
GTW26_RS21990 4697060..4697335 - 276 WP_023098713.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28220.34 Da        Isoelectric Point: 7.7294

>T147010 WP_003140635.1 NZ_CP047643:c4692995-4692240 [Pseudomonas aeruginosa CI27]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGAPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

>T147010 NZ_CP062851:714004-714300 [Escherichia coli O9:H10]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT147010 WP_003086329.1 NZ_CP047643:c4693498-4693130 [Pseudomonas aeruginosa CI27]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp

>AT147010 NZ_CP062851:714302-714697 [Escherichia coli O9:H10]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z7F3

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References