Detailed information of TA system TA147009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3703651..3704871
• Replicon: chromosome
• Accession: NZ_CP047643
• Organism: Pseudomonas aeruginosa CI27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: GTW26_RS17240
• Protein ID: WP_003110488.1
• Coordinates: 3704293..3704871 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GTW26_RS17235
• Protein ID: WP_003088112.1
• Coordinates: 3703651..3704205 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW26_RS17210 (GTW26_17185)	3699219..3699770	+	552	WP_003139878.1	XRE family transcriptional regulator	-
GTW26_RS17215 (GTW26_17190)	3699767..3700165	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
GTW26_RS17220 (GTW26_17195)	3700178..3700501	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
GTW26_RS17225 (GTW26_17200)	3700832..3701293	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
GTW26_RS17230 (GTW26_17205)	3701298..3703493	+	2196	WP_003139882.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GTW26_RS17235 (GTW26_17210)	3703651..3704205	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
GTW26_RS17240 (GTW26_17215)	3704293..3704871	+	579	WP_003110488.1	HD domain-containing protein	Toxin
GTW26_RS17245 (GTW26_17220)	3704980..3706167	-	1188	WP_003139884.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GTW26_RS17250 (GTW26_17225)	3706157..3708328	-	2172	WP_003139887.1	type I secretion system permease/ATPase	-
GTW26_RS17255 (GTW26_17230)	3708318..3709595	-	1278	WP_003139889.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T147009 WP_003110488.1 NZ_CP047643:3704293-3704871 [Pseudomonas aeruginosa CI27]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T147009 NZ_CP062851:652223-652537 [Escherichia coli O9:H10]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT147009 WP_003088112.1 NZ_CP047643:3703651-3704205 [Pseudomonas aeruginosa CI27]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT147009 NZ_CP062851:652534-652950 [Escherichia coli O9:H10]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4