Detailed information of TA system TA147007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1409277..1409947
• Replicon: chromosome
• Accession: NZ_CP047643
• Organism: Pseudomonas aeruginosa CI27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GTW26_RS06825
• Protein ID: WP_033868686.1
• Coordinates: 1409528..1409947 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GTW26_RS06820
• Protein ID: WP_033868685.1
• Coordinates: 1409277..1409531 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW26_RS06800 (GTW26_06780)	1405211..1406218	-	1008	WP_033868682.1	hypothetical protein	-
GTW26_RS06805 (GTW26_06785)	1406304..1406528	+	225	WP_009363662.1	AlpA family phage regulatory protein	-
GTW26_RS06810 (GTW26_06790)	1406531..1407409	+	879	WP_033868683.1	helicase RepA family protein	-
GTW26_RS06815 (GTW26_06795)	1407396..1408148	+	753	WP_033868684.1	hypothetical protein	-
GTW26_RS06820 (GTW26_06800)	1409277..1409531	+	255	WP_033868685.1	Arc family DNA-binding protein	Antitoxin
GTW26_RS06825 (GTW26_06805)	1409528..1409947	+	420	WP_033868686.1	type II toxin-antitoxin system VapC family toxin	Toxin
GTW26_RS06830 (GTW26_06810)	1410104..1410874	+	771	WP_033868687.1	P-type conjugative transfer protein TrbJ	-
GTW26_RS06835 (GTW26_06815)	1410885..1411043	+	159	WP_153570825.1	hypothetical protein	-
GTW26_RS06840 (GTW26_06820)	1411045..1412493	+	1449	WP_003137886.1	P-type conjugative transfer protein TrbL	-
GTW26_RS06845 (GTW26_06825)	1412562..1412804	+	243	WP_003137888.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
GTW26_RS06850 (GTW26_06830)	1412816..1413100	+	285	WP_003137890.1	hypothetical protein	-
GTW26_RS06855 (GTW26_06835)	1413103..1413471	+	369	WP_033868688.1	conjugal transfer transcriptional regulator TraJ	-
GTW26_RS06860 (GTW26_06840)	1413510..1413968	-	459	WP_065426345.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1405211..1422785	17574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14883.08 Da
• Isoelectric Point: 5.1890

>T147007 WP_033868686.1 NZ_CP047643:1409528-1409947 [Pseudomonas aeruginosa CI27]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYVSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T147007 NZ_CP062843:137349-137606 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGACGTATAGGAAATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.81 Da
• Isoelectric Point: 6.4760

>AT147007 WP_033868685.1 NZ_CP047643:1409277-1409531 [Pseudomonas aeruginosa CI27]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKAPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT147007 NZ_CP062843:137030-137260 [Escherichia coli]
ATGAGAACTGTATCCATTGTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA