Detailed information of TA system TA147005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 140994..141499
• Replicon: chromosome
• Accession: NZ_CP047643
• Organism: Pseudomonas aeruginosa CI27

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: GTW26_RS00645
• Protein ID: WP_003083773.1
• Coordinates: 140994..141275 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0H2ZJU8
• Locus tag: GTW26_RS00650
• Protein ID: WP_003137009.1
• Coordinates: 141272..141499 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW26_RS00620 (GTW26_00610)	136245..137594	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
GTW26_RS00625 (GTW26_00615)	137643..138329	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
GTW26_RS00630 (GTW26_00620)	138430..139164	+	735	WP_003110658.1	GntR family transcriptional regulator	-
GTW26_RS00635 (GTW26_00625)	139344..139754	+	411	WP_003110659.1	aegerolysin family protein	-
GTW26_RS00640 (GTW26_00630)	139786..140694	-	909	WP_003083769.1	LysR family transcriptional regulator	-
GTW26_RS00645 (GTW26_00635)	140994..141275	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
GTW26_RS00650 (GTW26_00640)	141272..141499	-	228	WP_003137009.1	CopG family ribbon-helix-helix protein	Antitoxin
GTW26_RS00655 (GTW26_00645)	141675..142298	-	624	WP_003137011.1	hypothetical protein	-
GTW26_RS00660 (GTW26_00650)	142399..142899	+	501	WP_003137013.1	LEA type 2 family protein	-
GTW26_RS00665 (GTW26_00655)	142971..143312	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
GTW26_RS00670 (GTW26_00660)	143394..144821	-	1428	WP_003137015.1	GABA permease	-
GTW26_RS00675 (GTW26_00665)	144990..146483	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T147005 WP_003083773.1 NZ_CP047643:c141275-140994 [Pseudomonas aeruginosa CI27]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T147005 NZ_CP062843:94750-95148 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.56 Da
• Isoelectric Point: 5.5740

>AT147005 WP_003137009.1 NZ_CP047643:c141499-141272 [Pseudomonas aeruginosa CI27]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHAEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT147005 NZ_CP062843:94523-94750 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A0H2ZJU8