Detailed information of TA system TA146999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 366..946
• Replicon: plasmid unnamed5
• Accession: NZ_CP047638
• Organism: Klebsiella pneumoniae strain K2606

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A060VNB1
• Locus tag: GUY22_RS29090
• Protein ID: WP_001136729.1
• Coordinates: 366..680 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: GUY22_RS29095
• Protein ID: WP_000093040.1
• Coordinates: 668..946 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY22_RS29090	366..680	-	315	WP_001136729.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GUY22_RS29095	668..946	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
GUY22_RS29100	1288..1506	-	219	WP_002193751.1	TonB family protein	-
GUY22_RS29105	1503..1874	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
GUY22_RS29110	2148..2393	-	246	WP_159210150.1	hypothetical protein	-
GUY22_RS29115	2720..4405	+	1686	WP_032440457.1	cloacin	-
GUY22_RS29120	4415..4672	+	258	WP_032440455.1	cloacin	-
GUY22_RS29125	4757..4906	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10077	10077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.77 Da
• Isoelectric Point: 9.8619

>T146999 WP_001136729.1 NZ_CP047638:c680-366 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKTLPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDR
EMDRIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T146999 NZ_CP062839:c106353-105973 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT146999 WP_000093040.1 NZ_CP047638:c946-668 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT146999 NZ_CP062839:c106574-106353 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A060VNB1

Antitoxin

• AlphaFold DB: A0A2X1PRM1