146990

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5234985..5235610 Replicon chromosome
Accession NZ_CP047633
Organism Klebsiella pneumoniae strain K2606

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GUY22_RS25770 Protein ID WP_002882817.1
Coordinates 5234985..5235368 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GUY22_RS25775 Protein ID WP_004150355.1
Coordinates 5235368..5235610 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUY22_RS25755 5232351..5233253 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GUY22_RS25760 5233250..5233885 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GUY22_RS25765 5233882..5234811 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
GUY22_RS25770 5234985..5235368 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GUY22_RS25775 5235368..5235610 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GUY22_RS25780 5235815..5236732 + 918 WP_009309725.1 alpha/beta hydrolase -
GUY22_RS25785 5236746..5237687 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GUY22_RS25790 5237732..5238169 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GUY22_RS25795 5238166..5239026 - 861 WP_004146232.1 virulence factor BrkB family protein -
GUY22_RS25800 5239020..5239619 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T146990 WP_002882817.1 NZ_CP047633:c5235368-5234985 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T146990 NZ_CP062838:2306426-2306713 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT146990 WP_004150355.1 NZ_CP047633:c5235610-5235368 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT146990 NZ_CP062838:2306187-2306426 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References