Detailed information of TA system TA146987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4633929..4634739
• Replicon: chromosome
• Accession: NZ_CP047633
• Organism: Klebsiella pneumoniae strain K2606

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GUY22_RS22905
• Protein ID: WP_101968539.1
• Coordinates: 4633929..4634462 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: GUY22_RS22910
• Protein ID: WP_002887278.1
• Coordinates: 4634473..4634739 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY22_RS22900	4632760..4633881	+	1122	WP_009309849.1	cupin domain-containing protein	-
GUY22_RS22905	4633929..4634462	-	534	WP_101968539.1	GNAT family N-acetyltransferase	Toxin
GUY22_RS22910	4634473..4634739	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
GUY22_RS22915	4634842..4636275	-	1434	WP_065782632.1	Cu(+)/Ag(+) sensor histidine kinase	-
GUY22_RS22920	4636265..4636948	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
GUY22_RS22925	4637121..4638506	+	1386	WP_135702016.1	efflux transporter outer membrane subunit	-
GUY22_RS22930	4638524..4638868	+	345	WP_032433444.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19826.69 Da
• Isoelectric Point: 5.2614

>T146987 WP_101968539.1 NZ_CP047633:c4634462-4633929 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLLSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T146987 NZ_CP062838:1847991-1848242 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT146987 WP_002887278.1 NZ_CP047633:c4634739-4634473 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT146987 NZ_CP062838:1848239-1848493 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLZ1