Detailed information of TA system TA146972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1300718..1301165
• Replicon: chromosome
• Accession: NZ_CP047628
• Organism: Lactococcus raffinolactis strain Lr_19_14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GU334_RS06400
• Protein ID: WP_167841368.1
• Coordinates: 1300899..1301165 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A5SDT8
• Locus tag: GU334_RS06395
• Protein ID: WP_061774359.1
• Coordinates: 1300718..1300909 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU334_RS06385	1295943..1297898	-	1956	WP_167840046.1	DNA topoisomerase IV subunit B	-
GU334_RS06390	1298299..1300452	-	2154	WP_096040472.1	type I DNA topoisomerase	-
GU334_RS06395	1300718..1300909	+	192	WP_061774359.1	hypothetical protein	Antitoxin
GU334_RS06400	1300899..1301165	+	267	WP_167841368.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GU334_RS06405	1301234..1302097	-	864	WP_061774360.1	ROK family protein	-
GU334_RS06410	1302103..1303527	-	1425	WP_167840048.1	glycoside hydrolase family 1 protein	-
GU334_RS06415	1303742..1304437	-	696	WP_167841369.1	LrgB family protein	-
GU334_RS06420	1304434..1304796	-	363	WP_061774363.1	CidA/LrgA family protein	-
GU334_RS06425	1304924..1306135	-	1212	WP_167841664.1	CapA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-87)

Antitoxin

• Protein of unknown function DUF6290 (2-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10537.16 Da
• Isoelectric Point: 9.7770

>T146972 WP_167841368.1 NZ_CP047628:1300899-1301165 [Lactococcus raffinolactis]
VTYDFIPSPKFNRQFRILDKFTQKCIQNYINNVILKADNPHNHGKQLVGDLAVLWHYRIGDYRLIVTIQDNELMVPALQI
NHRRDIYR

Nucleotide

• Length: 267 bp

>T146972 NZ_CP062836:15459-15740 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7328.24 Da
• Isoelectric Point: 4.1683

>AT146972 WP_061774359.1 NZ_CP047628:1300718-1300909 [Lactococcus raffinolactis]
MAKFEGLSLSESIKRTMVEKYENDYDLKVAETSIAEFQKYIISDKPVQSWEDLQPELGLDRDV

Nucleotide

• Length: 192 bp

>AT146972 NZ_CP062836:15193-15462 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5SDT8