Detailed information of TA system TA146970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1149678..1150125
• Replicon: chromosome
• Accession: NZ_CP047616
• Organism: Lactococcus raffinolactis strain Lr_19_5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GU336_RS05685
• Protein ID: WP_138491389.1
• Coordinates: 1149678..1149944 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A5SDT8
• Locus tag: GU336_RS05690
• Protein ID: WP_061774359.1
• Coordinates: 1149934..1150125 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU336_RS05660	1144708..1145919	+	1212	WP_096040552.1	CapA family protein	-
GU336_RS05665	1146047..1146409	+	363	WP_061774363.1	CidA/LrgA family protein	-
GU336_RS05670	1146406..1147101	+	696	WP_061774362.1	LrgB family protein	-
GU336_RS05675	1147316..1148740	+	1425	Protein_1076	glycoside hydrolase family 1 protein	-
GU336_RS05680	1148746..1149609	+	864	WP_061774360.1	ROK family protein	-
GU336_RS05685	1149678..1149944	-	267	WP_138491389.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GU336_RS05690	1149934..1150125	-	192	WP_061774359.1	hypothetical protein	Antitoxin
GU336_RS05695	1150391..1152544	+	2154	WP_167838662.1	type I DNA topoisomerase	-
GU336_RS05700	1152749..1153699	+	951	WP_167838195.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-87)

Antitoxin

• Protein of unknown function DUF6290 (2-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10586.30 Da
• Isoelectric Point: 10.0256

>T146970 WP_138491389.1 NZ_CP047616:c1149944-1149678 [Lactococcus raffinolactis]
VTYDFIPSPKFNRQFRILDKFTQKCIQNYINNVILKADNPHNHGKQLVGDLAVLWRYRIGDYRLIVMIQDNELMVPALQI
NHRRDIYR

Nucleotide

• Length: 267 bp

>T146970 NZ_CP062832:c4269317-4269144 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7328.24 Da
• Isoelectric Point: 4.1683

>AT146970 WP_061774359.1 NZ_CP047616:c1150125-1149934 [Lactococcus raffinolactis]
MAKFEGLSLSESIKRTMVEKYENDYDLKVAETSIAEFQKYIISDKPVQSWEDLQPELGLDRDV

Nucleotide

• Length: 192 bp

>AT146970 NZ_CP062832:c4269641-4269345 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5SDT8