Detailed information of TA system TA146969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 21693..22285
• Replicon: plasmid pNMBU_W06E18_Str1_3
• Accession: NZ_CP047612
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J7LNC2
• Locus tag: GU332_RS26025
• Protein ID: WP_000520550.1
• Coordinates: 21693..21941 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: GU332_RS26030
• Protein ID: WP_000681613.1
• Coordinates: 21938..22285 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS25995	18567..19082	+	516	WP_001025395.1	J domain-containing protein	-
GU332_RS26000	19187..19729	+	543	WP_001083907.1	hypothetical protein	-
GU332_RS26005	19726..19974	+	249	WP_001230706.1	hypothetical protein	-
GU332_RS26010	19964..20191	+	228	WP_000801440.1	hypothetical protein	-
GU332_RS26015	20645..20944	+	300	WP_000650312.1	hypothetical protein	-
GU332_RS26020	21038..21388	+	351	WP_000854257.1	hypothetical protein	-
GU332_RS26025	21693..21941	+	249	WP_000520550.1	type II toxin-antitoxin system HicA family toxin	Toxin
GU332_RS26030	21938..22285	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GU332_RS26035	22648..23151	+	504	WP_000781817.1	hypothetical protein	-
GU332_RS26040	23155..24384	+	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
GU332_RS26045	24461..24727	+	267	WP_000134734.1	hypothetical protein	-
GU332_RS26050	24732..24965	+	234	WP_000120384.1	EexN family lipoprotein	-
GU332_RS26055	25325..25945	+	621	WP_000157548.1	lytic transglycosylase domain-containing protein	-
GU332_RS26060	26013..26285	+	273	WP_001329503.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32448	32448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9437.05 Da
• Isoelectric Point: 10.4800

>T146969 WP_000520550.1 NZ_CP047612:21693-21941 [Escherichia coli]
MGKTDKLQAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T146969 NZ_CP062832:4255880-4256257 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT146969 WP_000681613.1 NZ_CP047612:21938-22285 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT146969 NZ_CP062832:4255456-4255833 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A6Y5WGJ7

Antitoxin

• AlphaFold DB: A0A829CLC1