Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 19726..20191 Replicon plasmid pNMBU_W06E18_Str1_3
Accession NZ_CP047612
Organism Escherichia coli strain NMBU_ W06E18

Toxin (Protein)


Gene name TsxA Uniprot ID M7C8T2
Locus tag GU332_RS26005 Protein ID WP_001230706.1
Coordinates 19726..19974 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag GU332_RS26010 Protein ID WP_000801440.1
Coordinates 19964..20191 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GU332_RS25980 15206..15733 - 528 WP_000577045.1 DUF2726 domain-containing protein -
GU332_RS25985 15869..16273 - 405 WP_001329491.1 hypothetical protein -
GU332_RS25990 16286..17152 - 867 WP_001353743.1 replication initiation protein -
GU332_RS25995 18567..19082 + 516 WP_001025395.1 J domain-containing protein -
GU332_RS26000 19187..19729 + 543 WP_001083907.1 hypothetical protein -
GU332_RS26005 19726..19974 + 249 WP_001230706.1 hypothetical protein Toxin
GU332_RS26010 19964..20191 + 228 WP_000801440.1 hypothetical protein Antitoxin
GU332_RS26015 20645..20944 + 300 WP_000650312.1 hypothetical protein -
GU332_RS26020 21038..21388 + 351 WP_000854257.1 hypothetical protein -
GU332_RS26025 21693..21941 + 249 WP_000520550.1 type II toxin-antitoxin system HicA family toxin -
GU332_RS26030 21938..22285 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
GU332_RS26035 22648..23151 + 504 WP_000781817.1 hypothetical protein -
GU332_RS26040 23155..24384 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
GU332_RS26045 24461..24727 + 267 WP_000134734.1 hypothetical protein -
GU332_RS26050 24732..24965 + 234 WP_000120384.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32448 32448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9594.94 Da        Isoelectric Point: 7.9925

>T146968 WP_001230706.1 NZ_CP047612:19726-19974 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ET

Download         Length: 249 bp

>T146968 NZ_CP062832:c3981114-3980827 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT146968 WP_000801440.1 NZ_CP047612:19964-20191 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT146968 NZ_CP062832:c3980840-3980538 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M7C8T2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References