Detailed information of TA system TA146962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5165273..5165760
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GU332_RS24945
• Protein ID: WP_001520461.1
• Coordinates: 5165509..5165760 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: GU332_RS24940
• Protein ID: WP_001520458.1
• Coordinates: 5165273..5165506 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS24915	5160347..5160634	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
GU332_RS24920	5160693..5162024	+	1332	WP_001520455.1	MFS transporter	-
GU332_RS24925	5162132..5162662	+	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GU332_RS24930	5162655..5164517	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
GU332_RS24935	5164708..5165187	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
GU332_RS24940	5165273..5165506	+	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GU332_RS24945	5165509..5165760	+	252	WP_001520461.1	CcdB family protein	Toxin
GU332_RS24950	5165820..5166668	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GU332_RS24955	5166675..5167052	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GU332_RS24960	5167055..5167876	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GU332_RS24965	5167873..5168862	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GU332_RS24970	5168862..5170148	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T146962 WP_001520461.1 NZ_CP047609:5165509-5165760 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T146962 NZ_CP062832:2306443-2306730 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT146962 WP_001520458.1 NZ_CP047609:5165273-5165506 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT146962 NZ_CP062832:2306204-2306443 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4