Detailed information of TA system TA146961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 5002105..5002919
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GU332_RS24165
• Protein ID: WP_001054376.1
• Coordinates: 5002662..5002919 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GU332_RS24160
• Protein ID: WP_001309181.1
• Coordinates: 5002105..5002650 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS24135	4997796..4999109	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
GU332_RS24140	4999121..4999399	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GU332_RS24145	4999396..5000517	-	1122	WP_172986673.1	M42 family metallopeptidase	-
GU332_RS24150	5000916..5001134	-	219	Protein_4726	hypothetical protein	-
GU332_RS24155	5001303..5002049	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GU332_RS24160	5002105..5002650	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GU332_RS24165	5002662..5002919	-	258	WP_001054376.1	hypothetical protein	Toxin
GU332_RS24170	5003296..5003541	-	246	Protein_4730	GNAT family N-acetyltransferase	-
GU332_RS24175	5003911..5004897	+	987	WP_001323209.1	hypothetical protein	-
GU332_RS24180	5005480..5006460	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GU332_RS24185	5006525..5007631	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T146961 WP_001054376.1 NZ_CP047609:c5002919-5002662 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T146961 NZ_CP062832:2020052-2020154 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT146961 WP_001309181.1 NZ_CP047609:c5002650-5002105 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT146961 NZ_CP062832:c2020192-2019921 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCACTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8