Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 1674578..1675104 | Replicon | chromosome |
| Accession | NZ_CP047609 | ||
| Organism | Escherichia coli strain NMBU_ W06E18 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1EXL1 |
| Locus tag | GU332_RS08025 | Protein ID | WP_000323025.1 |
| Coordinates | 1674578..1674865 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | GU332_RS08030 | Protein ID | WP_000534858.1 |
| Coordinates | 1674865..1675104 (-) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GU332_RS07980 | 1669662..1670120 | - | 459 | WP_000526135.1 | IS200/IS605 family transposase | - |
| GU332_RS07985 | 1670571..1670786 | - | 216 | WP_000066484.1 | cold shock-like protein CspB | - |
| GU332_RS07990 | 1671087..1671299 | + | 213 | WP_000087756.1 | cold shock-like protein CspF | - |
| GU332_RS07995 | 1671354..1671443 | + | 90 | WP_120795389.1 | hypothetical protein | - |
| GU332_RS08000 | 1671721..1672473 | - | 753 | WP_001047135.1 | antitermination protein | - |
| GU332_RS08005 | 1672487..1673536 | - | 1050 | WP_001265199.1 | DUF968 domain-containing protein | - |
| GU332_RS08010 | 1673538..1673816 | - | 279 | WP_012304870.1 | hypothetical protein | - |
| GU332_RS08015 | 1673883..1674134 | - | 252 | WP_000980994.1 | hypothetical protein | - |
| GU332_RS08020 | 1674351..1674506 | - | 156 | WP_000813254.1 | type I toxin-antitoxin system toxin HokD | - |
| GU332_RS08025 | 1674578..1674865 | - | 288 | WP_000323025.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| GU332_RS08030 | 1674865..1675104 | - | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| GU332_RS08035 | 1675129..1675434 | + | 306 | WP_001326990.1 | hypothetical protein | - |
| GU332_RS08040 | 1675637..1675969 | + | 333 | WP_001301033.1 | protein FlxA | - |
| GU332_RS08045 | 1676406..1677719 | - | 1314 | WP_000589005.1 | ISNCY family transposase | - |
| GU332_RS08050 | 1678203..1679225 | - | 1023 | WP_000915483.1 | DUF4868 domain-containing protein | - |
| GU332_RS08055 | 1679228..1679842 | - | 615 | WP_001265627.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1641685..1698072 | 56387 | |
| - | flank | IS/Tn | - | - | 1669662..1670120 | 458 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11225.22 Da Isoelectric Point: 10.1967
>T146940 WP_000323025.1 NZ_CP047609:c1674865-1674578 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T146940 NZ_CP062826:4255056-4255433 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT146940 WP_000534858.1 NZ_CP047609:c1675104-1674865 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT146940 NZ_CP062826:4254632-4255009 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|