Detailed information of TA system TA146939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1581727..1582365
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: GU332_RS07555
• Protein ID: WP_001306887.1
• Coordinates: 1582189..1582365 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GU332_RS07550
• Protein ID: WP_001270286.1
• Coordinates: 1581727..1582143 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS07530	1576879..1577820	-	942	WP_001251315.1	ABC transporter permease	-
GU332_RS07535	1577821..1578834	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
GU332_RS07540	1578852..1579997	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
GU332_RS07545	1580242..1581648	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
GU332_RS07550	1581727..1582143	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GU332_RS07555	1582189..1582365	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GU332_RS07560	1582587..1582817	+	231	WP_000494241.1	YncJ family protein	-
GU332_RS07565	1582909..1584870	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GU332_RS07570	1584943..1585479	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
GU332_RS07575	1585532..1586743	+	1212	WP_023143671.1	BenE family transporter YdcO	-
GU332_RS07580	1586851..1587309	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1586851..1587309	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T146939 WP_001306887.1 NZ_CP047609:c1582365-1582189 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T146939 NZ_CP062826:c3980290-3980003 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT146939 WP_001270286.1 NZ_CP047609:c1582143-1581727 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT146939 NZ_CP062826:c3980016-3979714 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76