Detailed information of TA system TA146924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4854785..4855401
• Replicon: chromosome
• Accession: NZ_CP047606
• Organism: Citrobacter sp. LUTT5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0J1MQ96
• Locus tag: GUC46_RS23070
• Protein ID: WP_003028682.1
• Coordinates: 4854785..4855159 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GUC46_RS23075
• Protein ID: WP_161805918.1
• Coordinates: 4855159..4855401 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUC46_RS23055	4852288..4853190	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
GUC46_RS23060	4853187..4853822	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
GUC46_RS23065	4853819..4854748	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
GUC46_RS23070	4854785..4855159	-	375	WP_003028682.1	type II toxin-antitoxin system VapC family toxin	Toxin
GUC46_RS23075	4855159..4855401	-	243	WP_161805918.1	ribbon-helix-helix domain-containing protein	Antitoxin
GUC46_RS23080	4855607..4856515	+	909	WP_003840443.1	alpha/beta hydrolase	-
GUC46_RS23085	4856666..4857607	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
GUC46_RS23090	4857652..4858089	-	438	WP_044714708.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUC46_RS23095	4858086..4858958	-	873	WP_003028669.1	virulence factor BrkB family protein	-
GUC46_RS23100	4858952..4859551	-	600	WP_016151258.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13674.89 Da
• Isoelectric Point: 9.5622

>T146924 WP_003028682.1 NZ_CP047606:c4855159-4854785 [Citrobacter sp. LUTT5]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Nucleotide

• Length: 375 bp

>T146924 NZ_CP062826:c618131-617667 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9523.81 Da
• Isoelectric Point: 4.2805

>AT146924 WP_161805918.1 NZ_CP047606:c4855401-4855159 [Citrobacter sp. LUTT5]
MMAEMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT146924 NZ_CP062826:c618466-618131 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0J1MQ96