Detailed information of TA system TA146922

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4324324..4325027
• Replicon: chromosome
• Accession: NZ_CP047606
• Organism: Citrobacter sp. LUTT5

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GUC46_RS20750
• Protein ID: WP_032728834.1
• Coordinates: 4324324..4324665 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GUC46_RS20755
• Protein ID: WP_096810298.1
• Coordinates: 4324686..4325027 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUC46_RS20730	4320814..4321176	+	363	WP_044713046.1	endoribonuclease SymE	-
GUC46_RS20735	4321229..4321864	-	636	WP_121540722.1	HNH endonuclease	-
GUC46_RS20740	4322005..4322877	+	873	WP_121540721.1	HNH endonuclease	-
GUC46_RS20745	4323058..4324065	-	1008	WP_049017154.1	restriction endonuclease	-
GUC46_RS20750	4324324..4324665	-	342	WP_032728834.1	TA system toxin CbtA family protein	Toxin
GUC46_RS20755	4324686..4325027	-	342	WP_096810298.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GUC46_RS20760	4325038..4325580	-	543	WP_096810296.1	DNA repair protein RadC	-
GUC46_RS20765	4325593..4326036	-	444	WP_047667661.1	antirestriction protein	-
GUC46_RS20770	4326067..4326888	-	822	WP_160180667.1	DUF945 domain-containing protein	-
GUC46_RS20775	4327009..4327482	-	474	WP_201743313.1	hypothetical protein	-
GUC46_RS20780	4327584..4328048	-	465	WP_114262839.1	hypothetical protein	-
GUC46_RS20785	4328357..4329232	-	876	WP_160180669.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12777.71 Da
• Isoelectric Point: 7.1648

>T146922 WP_032728834.1 NZ_CP047606:c4324665-4324324 [Citrobacter sp. LUTT5]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T146922 NZ_CP062825:59791-59943 [Escherichia coli O167:H26]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12758.31 Da
• Isoelectric Point: 5.0491

>AT146922 WP_096810298.1 NZ_CP047606:c4325027-4324686 [Citrobacter sp. LUTT5]
MKSTGSENDSFLQWGLNRNVTPCFGARLVQEGNQLHYLADRVSITGEFSNAESLKLDEVFPRFISQMESMLTTSEMNPRY
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT146922 NZ_CP062825:c59736-59679 [Escherichia coli O167:H26]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT