Detailed information of TA system TA146895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2150174..2150801
• Replicon: chromosome
• Accession: NZ_CP047602
• Organism: Thermoanaerobacterium aotearoense strain SCUT27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3VUG0
• Locus tag: GSH73_RS10615
• Protein ID: WP_014758038.1
• Coordinates: 2150174..2150524 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I3VUF9
• Locus tag: GSH73_RS10620
• Protein ID: WP_013297076.1
• Coordinates: 2150526..2150801 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSH73_RS10595	2145622..2146353	-	732	WP_038070092.1	WecB/TagA/CpsF family glycosyltransferase	-
GSH73_RS10600	2146356..2147435	-	1080	WP_014758041.1	polysaccharide pyruvyl transferase CsaB	-
GSH73_RS10605	2147473..2149494	-	2022	WP_014758040.1	hypothetical protein	-
GSH73_RS10610	2149608..2150057	-	450	WP_014758039.1	hypothetical protein	-
GSH73_RS10615	2150174..2150524	-	351	WP_014758038.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GSH73_RS10620	2150526..2150801	-	276	WP_013297076.1	ribbon-helix-helix protein, CopG family	Antitoxin
GSH73_RS10625	2150889..2152055	-	1167	WP_014758037.1	alanine racemase	-
GSH73_RS10630	2152069..2153052	-	984	WP_038070095.1	outer membrane lipoprotein carrier protein LolA	-
GSH73_RS10635	2153066..2154592	-	1527	WP_014758035.1	NAD(P)H-hydrate dehydratase	-
GSH73_RS10640	2154670..2155034	-	365	Protein_2091	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12931.00 Da
• Isoelectric Point: 6.4714

>T146895 WP_014758038.1 NZ_CP047602:c2150524-2150174 [Thermoanaerobacterium aotearoense]
MIVKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T146895 NZ_CP062821:c3154854-3154444 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACTCAATTGTTCTCAACCTGTGTTCATGGGGTTGTTGGGAT
GTTTTTACTATTGGCTCCATGGTCCCCTGATAACTCAATGATATGGTTGCCGCTATTAGTGGTTGTCGTTGCGAGTTGGG
CAAAGAGCCAGAAAAATATCAGTAAAATAAAAGGGGTTGCTGTACTGGTTAATGGCAATAAGGTTCAATGGAAGAAAAAT
GAGTGGAGAATACTGAAAGCGCCTTGGCTTAGCCGTTATGGGGTATTGCTGACACTAGAAGCTTTGCAAGGTAAACCGCA
AAAGGTTCATTTATGGATAGCGAAAGATGCTGTGTCAGAAGAGAATTGGCGTAATTTAAACCAGTTATTACTGCAATACC
CTGATATCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10514.31 Da
• Isoelectric Point: 7.7813

>AT146895 WP_013297076.1 NZ_CP047602:c2150801-2150526 [Thermoanaerobacterium aotearoense]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Nucleotide

• Length: 276 bp

>AT146895 NZ_CP062821:c3155101-3154835 [Providencia rettgeri]
ATGGATATTGAGAATAAAGCGCGAATTCATTGGGCATGCCGTCGTGGTATGCGTGAATTAGATATTTCAATCATGCCGTT
TTTTGAGTATGAATATGATGACCTAACAACTCACGATAAGCAGGTATTTGTCCGTTTATTAGAATGTGCGGACCCTGATT
TATTCAATTGGTTAATGAATCATGGTCGCCCAGATGATGACGAACTTTTTCGTATGATTAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• AlphaFold DB: I3VUG0

Antitoxin

• AlphaFold DB: I3VUF9