Detailed information of TA system TA146890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 117241..117866
• Replicon: plasmid pTrh52_1
• Accession: NZ_CP047601
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GUK18_RS23420
• Protein ID: WP_021572485.1
• Coordinates: 117468..117866 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: GUK18_RS23415
• Protein ID: WP_000450532.1
• Coordinates: 117241..117468 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS23415	117241..117468	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GUK18_RS23420	117468..117866	+	399	WP_021572485.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GUK18_RS23425	117875..120028	-	2154	WP_104934212.1	type IV conjugative transfer system coupling protein TraD	-
GUK18_RS23430	120281..121012	-	732	WP_024244211.1	complement resistance protein TraT	-
GUK18_RS23435	121044..121541	-	498	WP_021572404.1	surface exclusion inner membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..158248	158248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14858.13 Da
• Isoelectric Point: 7.8605

>T146890 WP_021572485.1 NZ_CP047601:117468-117866 [Escherichia coli O84:H7]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNDTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T146890 NZ_CP062821:c367512-367150 [Providencia rettgeri]
GTGTGGATAGTCATATTAACACCTCGTTTTGAAACCTGGCTAAACAAACAAAATGGGCGCACTCAAGAAAAAATTTTAGC
TGCACTTAGAAATTTAGAAGTTTTTGGCCCAAGATTGCCTCGCCCCTATGCCGATACAATAAAAGGCTCTAAGTACAGCA
GCATGAAAGAACTCAGGGTTCAACATGCAGGCAAAGCCCTCCGCGCATTTTTTGTATTTGATATTCAGCGGCAAGCGATT
GTTTTATGTGGTGGTGATAAAAGTAATGACAAACTTTTTTATCAAACTATGGTACGAATCGCGGATGATGAGTTTACTGC
ATATCTAAAATTGATGGAGAAAAAAAATGAAGAGCCTACATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT146890 WP_000450532.1 NZ_CP047601:117241-117468 [Escherichia coli O84:H7]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT146890 NZ_CP062821:c367166-366867 [Providencia rettgeri]
ATGAAGAGCCTACATGATGTAATTGCTGAACGCTGCCCAGAAAGCCAAGCAAGAATTAACGAGTTAGCGGATGAAATGAT
ACTTGCAACGGGGCTTCAGTTAATGCGAGAGGAACTTAATATTTCGCAAAAGATGCTAGCCAGCCGCCTTGGTATTTCTC
AACCAGCTATTACTCAAATTGAACAACGAGGCAATGATCTTAAACTGGCAACATTAAAACGCTATGTTGAAGCAATTGGA
GGTAAACTCAGCCTTACGATTGAATTACCGACAGGTAATAGCCGAGTCTTTCATATCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5