Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 78425..78950 Replicon plasmid pTrh52_1
Accession NZ_CP047601
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GUK18_RS23215 Protein ID WP_001159871.1
Coordinates 78425..78730 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag GUK18_RS23220 Protein ID WP_000813639.1
Coordinates 78732..78950 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS23190 74315..74986 - 672 WP_024244225.1 response regulator transcription factor HprR -
GUK18_RS23195 75118..75531 + 414 WP_021541665.1 hydroxyisourate hydrolase -
GUK18_RS23200 75630..76622 + 993 WP_021541666.1 protein-methionine-sulfoxide reductase catalytic subunit MsrP -
GUK18_RS23205 76623..77258 + 636 WP_021541667.1 protein-methionine-sulfoxide reductase heme-binding subunit MsrQ -
GUK18_RS23210 77618..78424 - 807 WP_021541668.1 site-specific integrase -
GUK18_RS23215 78425..78730 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GUK18_RS23220 78732..78950 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GUK18_RS23225 79547..79804 + 258 WP_021564233.1 hypothetical protein -
GUK18_RS23230 79804..80394 + 591 WP_021564232.1 hypothetical protein -
GUK18_RS23235 80777..81955 - 1179 WP_021572468.1 hypothetical protein -
GUK18_RS23240 82003..82518 - 516 WP_021572469.1 type 1 fimbrial protein -
GUK18_RS23245 82585..83271 - 687 WP_021572470.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC 1..158248 158248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T146887 WP_001159871.1 NZ_CP047601:c78730-78425 [Escherichia coli O84:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T146887 NZ_CP062820:c133273-132968 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT146887 WP_000813639.1 NZ_CP047601:c78950-78732 [Escherichia coli O84:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT146887 NZ_CP062820:c133493-133275 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References