Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4290453..4291071 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GUK18_RS20510 Protein ID WP_001291435.1
Coordinates 4290453..4290671 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GUK18_RS20515 Protein ID WP_000344800.1
Coordinates 4290697..4291071 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS20475 4285741..4286313 + 573 WP_000779831.1 YbaY family lipoprotein -
GUK18_RS20480 4286344..4286655 - 312 WP_000409908.1 MGMT family protein -
GUK18_RS20490 4287034..4287387 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GUK18_RS20495 4287429..4288979 - 1551 WP_001524176.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GUK18_RS20500 4289143..4289613 - 471 WP_000136192.1 YlaC family protein -
GUK18_RS20505 4289729..4290280 - 552 WP_000102539.1 maltose O-acetyltransferase -
GUK18_RS20510 4290453..4290671 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GUK18_RS20515 4290697..4291071 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GUK18_RS20520 4291616..4294765 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
GUK18_RS20525 4294788..4295981 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146885 WP_001291435.1 NZ_CP047600:c4290671-4290453 [Escherichia coli O84:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146885 NZ_CP062820:c78743-78537 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146885 WP_000344800.1 NZ_CP047600:c4291071-4290697 [Escherichia coli O84:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146885 NZ_CP062820:78730-78791 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References