Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yeeU/CbtA-CbeA
Location 3691186..3691893 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name ykfI Uniprot ID A0A4T3Q6D2
Locus tag GUK18_RS17750 Protein ID WP_001683515.1
Coordinates 3691552..3691893 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3L5H4Q7
Locus tag GUK18_RS17745 Protein ID WP_029696721.1
Coordinates 3691186..3691521 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS17720 3687132..3687350 - 219 WP_001064742.1 AlpA family phage regulatory protein -
GUK18_RS17725 3687468..3688082 - 615 WP_000772910.1 inovirus Gp2 family protein -
GUK18_RS17730 3688675..3689628 + 954 WP_024223205.1 hypothetical protein -
GUK18_RS17735 3689865..3690683 + 819 WP_024223204.1 DUF945 domain-containing protein -
GUK18_RS17740 3690713..3691186 + 474 WP_001552321.1 DNA repair protein RadC -
GUK18_RS17745 3691186..3691521 + 336 WP_029696721.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GUK18_RS17750 3691552..3691893 + 342 WP_001683515.1 TA system toxin CbtA family protein Toxin
GUK18_RS17755 3692008..3692726 + 719 Protein_3463 DUF4942 domain-containing protein -
GUK18_RS17760 3692803..3693051 + 249 WP_024187601.1 ribbon-helix-helix domain-containing protein -
GUK18_RS17765 3693055..3693330 + 276 WP_001683517.1 type II toxin-antitoxin system RelE/ParE family toxin -
GUK18_RS17770 3693444..3694241 + 798 WP_024223201.1 helix-turn-helix transcriptional regulator -
GUK18_RS17775 3694571..3695836 + 1266 WP_054486255.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC 3673495..3724543 51048


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-110)

Antitoxin

Antitoxin CbeA

(10-110)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13034.05 Da        Isoelectric Point: 9.5454

>T146883 WP_001683515.1 NZ_CP047600:3691552-3691893 [Escherichia coli O84:H7]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Download         Length: 342 bp

>T146883 NZ_CP062819:65159-65311 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12572.62 Da        Isoelectric Point: 7.6388

>AT146883 WP_029696721.1 NZ_CP047600:3691186-3691521 [Escherichia coli O84:H7]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGCCGYVYIAIYPTQR

Download         Length: 336 bp

>AT146883 NZ_CP062819:c65104-65047 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4T3Q6D2

Antitoxin

Source ID Structure
AlphaFold DB A0A3L5H4Q7

References