Detailed information of TA system TA146880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2686111..2686706
• Replicon: chromosome
• Accession: NZ_CP047600
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A788NS14
• Locus tag: GUK18_RS13045
• Protein ID: WP_024223020.1
• Coordinates: 2686111..2686488 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: GUK18_RS13050
• Protein ID: WP_000557315.1
• Coordinates: 2686485..2686706 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS13025	2681687..2682757	-	1071	WP_000907827.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
GUK18_RS13030	2682759..2683604	-	846	WP_000572183.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GUK18_RS13035	2683601..2684488	-	888	WP_000099282.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GUK18_RS13040	2684586..2685902	-	1317	WP_021581115.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GUK18_RS13045	2686111..2686488	-	378	WP_024223020.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GUK18_RS13050	2686485..2686706	-	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GUK18_RS13055	2686794..2687507	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GUK18_RS13060	2687509..2688276	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GUK18_RS13065	2688273..2689550	-	1278	WP_024223021.1	branched chain amino acid ABC transporter permease LivM	-
GUK18_RS13070	2689547..2690473	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GUK18_RS13075	2690521..2691630	-	1110	WP_001297972.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2680947..2689550	8603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13980.29 Da
• Isoelectric Point: 6.7263

>T146880 WP_024223020.1 NZ_CP047600:c2686488-2686111 [Escherichia coli O84:H7]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDLGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T146880 NZ_CP047600:c2686488-2686111 [Escherichia coli O84:H7]
ATGACCATTCAGTTTATCTCAGCGGAGGAAATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCTAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGTGTTTCTGATCTTT
GGCTGCTGGCGGCGATGCATTTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT146880 WP_000557315.1 NZ_CP047600:c2686706-2686485 [Escherichia coli O84:H7]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT146880 NZ_CP047600:c2686706-2686485 [Escherichia coli O84:H7]
ATGCATACTATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A788NS14

Antitoxin

• AlphaFold DB: D6JG32