Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2100675..2101329 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GUK18_RS10125 Protein ID WP_000244781.1
Coordinates 2100675..2101082 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GUK18_RS10130 Protein ID WP_000354046.1
Coordinates 2101063..2101329 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS10105 2096632..2098365 - 1734 WP_024235067.1 single-stranded-DNA-specific exonuclease RecJ -
GUK18_RS10110 2098371..2099081 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GUK18_RS10115 2099106..2100002 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GUK18_RS10120 2100114..2100635 + 522 WP_001055874.1 flavodoxin FldB -
GUK18_RS10125 2100675..2101082 - 408 WP_000244781.1 protein YgfX Toxin
GUK18_RS10130 2101063..2101329 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GUK18_RS10135 2101572..2102552 + 981 WP_024223065.1 tRNA-modifying protein YgfZ -
GUK18_RS10140 2102629..2103288 - 660 WP_000250274.1 hemolysin III family protein -
GUK18_RS10145 2103452..2103763 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
GUK18_RS10150 2103808..2105241 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T146878 WP_000244781.1 NZ_CP047600:c2101082-2100675 [Escherichia coli O84:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T146878 NZ_CP062815:4254531-4254908 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT146878 WP_000354046.1 NZ_CP047600:c2101329-2101063 [Escherichia coli O84:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT146878 NZ_CP062815:4254107-4254484 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References