Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1167458..1168289 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag GUK18_RS05800 Protein ID WP_000854814.1
Coordinates 1167915..1168289 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag GUK18_RS05795 Protein ID WP_001285585.1
Coordinates 1167458..1167826 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS05760 1162696..1163766 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
GUK18_RS05765 1163902..1164579 + 678 WP_001362823.1 hypothetical protein -
GUK18_RS05770 1164595..1165005 + 411 WP_203164440.1 hypothetical protein -
GUK18_RS05775 1165226..1166047 + 822 WP_001234530.1 DUF945 domain-containing protein -
GUK18_RS05780 1166129..1166608 + 480 WP_000860076.1 antirestriction protein -
GUK18_RS05785 1166624..1167100 + 477 WP_001186773.1 RadC family protein -
GUK18_RS05790 1167163..1167384 + 222 WP_000692323.1 DUF987 domain-containing protein -
GUK18_RS05795 1167458..1167826 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
GUK18_RS05800 1167915..1168289 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
GUK18_RS05805 1168286..1168480 + 195 WP_000988600.1 hypothetical protein -
GUK18_RS05810 1168493..1168606 + 114 WP_001161660.1 DUF957 domain-containing protein -
GUK18_RS05815 1168895..1169023 - 129 Protein_1144 transposase domain-containing protein -
GUK18_RS05820 1169095..1169277 + 183 WP_001016348.1 hypothetical protein -
GUK18_RS05825 1169378..1169707 - 330 WP_000450409.1 DUF496 family protein -
GUK18_RS05830 1169879..1170937 - 1059 WP_001200891.1 FUSC family protein -
GUK18_RS05835 1171135..1171608 - 474 WP_001105414.1 DNA gyrase inhibitor SbmC -
GUK18_RS05840 1171727..1172893 - 1167 WP_001442313.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T146875 WP_000854814.1 NZ_CP047600:1167915-1168289 [Escherichia coli O84:H7]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T146875 NZ_CP062815:3445317-3445535 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT146875 WP_001285585.1 NZ_CP047600:1167458-1167826 [Escherichia coli O84:H7]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT146875 NZ_CP062815:3444917-3445291 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References