Detailed information of TA system TA146860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 9132..9679
• Replicon: plasmid pKpn1693-CTXM
• Accession: NZ_CP047597
• Organism: Klebsiella pneumoniae strain Kpn 1693

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GTK25_RS26545
• Protein ID: WP_244449344.1
• Coordinates: 9401..9679 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: D2U5P9
• Locus tag: GTK25_RS26540
• Protein ID: WP_012917666.1
• Coordinates: 9132..9401 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK25_RS26505 (4761)	4761..4973	+	213	WP_001309245.1	ANR family transcriptional regulator	-
GTK25_RS26510 (5218)	5218..5679	+	462	WP_001233838.1	thermonuclease family protein	-
GTK25_RS26515 (5725)	5725..5934	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
GTK25_RS26520 (5972)	5972..6562	+	591	WP_000766807.1	DUF2726 domain-containing protein	-
GTK25_RS26525 (6802)	6802..7056	+	255	WP_000083830.1	replication regulatory protein RepA	-
GTK25_RS26530 (7294)	7294..7368	+	75	WP_001365705.1	RepA leader peptide Tap	-
GTK25_RS26535 (7361)	7361..8218	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
GTK25_RS26540 (9132)	9132..9401	+	270	WP_012917666.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
GTK25_RS26545 (9401)	9401..9679	+	279	WP_244449344.1	type II toxin-antitoxin system toxin YacB	Toxin
GTK25_RS26550 (9725)	9725..10573	+	849	WP_012881117.1	3'-5' exonuclease	-
GTK25_RS26555 (10690)	10690..11172	+	483	WP_001311056.1	hypothetical protein	-
GTK25_RS26560 (11207)	11207..12229	-	1023	Protein_15	maltoporin LamB	-
GTK25_RS27170 (12278)	12278..12730	-	453	Protein_16	IS5 family transposase	-
GTK25_RS26565 (12765)	12765..13640	-	876	WP_012579082.1	extended-spectrum class A beta-lactamase CTX-M-24	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..70307	70307
	-	flank	IS/Tn	blaCTX-M-14	-	12765..15152	2387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10744.34 Da
• Isoelectric Point: 8.4615

>T146860 WP_244449344.1 NZ_CP047597:9401-9679 [Klebsiella pneumoniae]
MEIFWTILASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T146860 NZ_CP062813:58678-58839 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10153.49 Da
• Isoelectric Point: 7.1674

>AT146860 WP_012917666.1 NZ_CP047597:9132-9401 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGCQQLERGDVLPHDMVESSATAWRD
EISRKVAGK

Nucleotide

• Length: 270 bp

>AT146860 NZ_CP062813:58414-58625 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin

• AlphaFold DB: D2U5P9