Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 50296..51206 Replicon plasmid pKpn1693-Vir
Accession NZ_CP047596
Organism Klebsiella pneumoniae strain Kpn 1693

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag GTK25_RS25715 Protein ID WP_004026354.1
Coordinates 50296..50766 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag GTK25_RS25720 Protein ID WP_004026357.1
Coordinates 50763..51206 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK25_RS25695 (GTK25_25325) 45708..46106 - 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -
GTK25_RS25700 (GTK25_25330) 46443..46937 - 495 WP_004212794.1 thermonuclease family protein -
GTK25_RS27040 47115..47381 + 267 WP_223175074.1 DUF1173 family protein -
GTK25_RS25705 (GTK25_25335) 47414..48064 + 651 WP_068893702.1 DUF1173 family protein -
GTK25_RS25715 (GTK25_25345) 50296..50766 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
GTK25_RS25720 (GTK25_25350) 50763..51206 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
GTK25_RS25725 (GTK25_25355) 51307..51810 - 504 Protein_62 DUF4113 domain-containing protein -
GTK25_RS25730 (GTK25_25360) 51908..52129 - 222 WP_004213643.1 DUF2188 domain-containing protein -
GTK25_RS25735 (GTK25_25365) 52310..52429 - 120 Protein_64 Fe3+-citrate ABC transporter substrate-binding protein -
GTK25_RS25740 (GTK25_25370) 52504..54630 - 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
GTK25_RS25745 (GTK25_25375) 54717..55670 - 954 Protein_66 fec operon regulator FecR -
GTK25_RS25750 (GTK25_25380) 55667..56178 - 512 Protein_67 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..192347 192347
- flank IS/Tn - - 49450..49953 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T146858 WP_004026354.1 NZ_CP047596:c50766-50296 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T146858 NZ_CP062812:59791-59943 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT146858 WP_004026357.1 NZ_CP047596:c51206-50763 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT146858 NZ_CP062812:c59736-59679 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References