Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5253363..5253988 Replicon chromosome
Accession NZ_CP047595
Organism Klebsiella pneumoniae strain Kpn 1693

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag GTK25_RS25065 Protein ID WP_012737091.1
Coordinates 5253363..5253746 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GTK25_RS25070 Protein ID WP_004150355.1
Coordinates 5253746..5253988 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK25_RS25050 (5250729) 5250729..5251631 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GTK25_RS25055 (5251628) 5251628..5252263 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GTK25_RS25060 (5252260) 5252260..5253189 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GTK25_RS25065 (5253363) 5253363..5253746 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
GTK25_RS25070 (5253746) 5253746..5253988 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GTK25_RS25075 (5254193) 5254193..5255110 + 918 WP_012737090.1 alpha/beta hydrolase -
GTK25_RS25080 (5255124) 5255124..5256065 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GTK25_RS25085 (5256110) 5256110..5256547 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GTK25_RS25090 (5256544) 5256544..5257404 - 861 WP_004146232.1 virulence factor BrkB family protein -
GTK25_RS25095 (5257398) 5257398..5257997 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T146857 WP_012737091.1 NZ_CP047595:c5253746-5253363 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T146857 NZ_CP062812:15459-15740 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT146857 WP_004150355.1 NZ_CP047595:c5253988-5253746 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT146857 NZ_CP062812:15193-15462 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References