Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4616184..4616994 Replicon chromosome
Accession NZ_CP047595
Organism Klebsiella pneumoniae strain Kpn 1693

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag GTK25_RS22090 Protein ID WP_014908042.1
Coordinates 4616184..4616717 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GTK25_RS22095 Protein ID WP_002887278.1
Coordinates 4616728..4616994 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK25_RS22085 (4615015) 4615015..4616136 + 1122 WP_012737335.1 cupin domain-containing protein -
GTK25_RS22090 (4616184) 4616184..4616717 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
GTK25_RS22095 (4616728) 4616728..4616994 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
GTK25_RS22100 (4617097) 4617097..4618530 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
GTK25_RS22105 (4618520) 4618520..4619203 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
GTK25_RS22110 (4619375) 4619375..4620760 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
GTK25_RS22115 (4620778) 4620778..4621122 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T146855 WP_014908042.1 NZ_CP047595:c4616717-4616184 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T146855 NZ_CP062811:c4410509-4410159 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT146855 WP_002887278.1 NZ_CP047595:c4616994-4616728 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT146855 NZ_CP062811:c4410754-4410503 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References