Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3500094..3500677 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag GUU88_RS16990 Protein ID WP_000254738.1
Coordinates 3500094..3500429 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GUU88_RS16995 Protein ID WP_000581937.1
Coordinates 3500429..3500677 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS16975 3495981..3497279 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GUU88_RS16980 3497367..3499004 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GUU88_RS16985 3499232..3500023 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GUU88_RS16990 3500094..3500429 - 336 WP_000254738.1 endoribonuclease MazF Toxin
GUU88_RS16995 3500429..3500677 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GUU88_RS17000 3500755..3502989 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GUU88_RS17005 3503037..3504338 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T146837 WP_000254738.1 NZ_CP047594:c3500429-3500094 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T146837 NZ_CP062811:303643-303930 [Escherichia coli]
ATAAGGACATGCCATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAACACATTGGATCAAATATTTAAATCTCC
TGTTCCTCAAGGGATTAAGTGGTCGGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTT
CGCGTTGTAAGTTCATACTAAATATGAGCGCTGCGTGTTTCCATCGGCCTCACCCGTCACCGGATACCGATAAAGGCGCT
GTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT146837 WP_000581937.1 NZ_CP047594:c3500677-3500429 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT146837 NZ_CP062811:303927-304286 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References