Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2104733..2105371 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GUU88_RS10255 Protein ID WP_000813794.1
Coordinates 2104733..2104909 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GUU88_RS10260 Protein ID WP_001270286.1
Coordinates 2104955..2105371 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS10235 2100352..2101566 - 1215 WP_001326689.1 BenE family transporter YdcO -
GUU88_RS10240 2101619..2102155 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GUU88_RS10245 2102228..2104189 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GUU88_RS10250 2104281..2104511 - 231 WP_000494244.1 YncJ family protein -
GUU88_RS10255 2104733..2104909 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GUU88_RS10260 2104955..2105371 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GUU88_RS10265 2105450..2106856 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GUU88_RS10270 2107101..2108246 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GUU88_RS10275 2108264..2109277 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GUU88_RS10280 2109278..2110219 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T146825 WP_000813794.1 NZ_CP047594:2104733-2104909 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T146825 NZ_CP062803:318474-318776 [Cupriavidus basilensis]
ATGGAGAAACATACGCCTCACTGCAGGCTCTCGGTGATCAGGACCTTGGCCATCGAAGGGCGGGTTTGCCTGACCAGCTC
CGCTGCCGTGCACAGTCGCAAGCTCGGATTCGACAGTGCCGACGTGATCGCCGTCATTGCTTCGCCGACGTCGGCAGACT
TTTACAAGAGCATGACGACCTACGCGGATCACAAGGTATGGCAGGACGTTTACCGGCCGATTACCGCCGCCGGCGCACTG
TATCTGAAACTGACGGTCACCGATGCGGTGCTGGTCGTGTCATTCAAGGAGCTGGAACCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT146825 WP_001270286.1 NZ_CP047594:2104955-2105371 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT146825 NZ_CP062803:318773-319174 [Cupriavidus basilensis]
ATGAAATGTCCAGAATGCGGGGGTGCGGAACTGGTGCAGGACACGCGCGACGTGCCTTATACGTACAAGGGCGAAACTGT
CATCATTTCCGCGGTCTCGGGCGGCTTTTGTCCGGCGTGCGGGGAAATCGTGCTGGATCCGAATCAAGCGGACCGGTACG
GCGAAGCGGCGGTGGCTTTCCAAAAGCAGGTCAATGCCGCCATCGTCGATCCCGCCTTCATCGTGGCCGTGCGCAAGAAG
CTGAGCCTGGACCAGCGCGAGGCCGCCGAGATATTCGGCGGCGGCGTCAATGCCTTCTCGCGCTACGAAACCGGCAAGAC
CAAGCCGCCGCTGGCGCTGGTCAAGCTGCTCAAGGTGCTGGAGCGGCACCCGGAGCTGCTTGATGAGGTGCGCTCAGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References