Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1087504..1088122 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GUU88_RS05335 Protein ID WP_001291435.1
Coordinates 1087504..1087722 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GUU88_RS05340 Protein ID WP_000344800.1
Coordinates 1087748..1088122 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS05300 1082793..1083365 + 573 WP_000779842.1 YbaY family lipoprotein -
GUU88_RS05305 1083396..1083707 - 312 WP_000409911.1 MGMT family protein -
GUU88_RS05315 1084086..1084439 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GUU88_RS05320 1084481..1086031 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GUU88_RS05325 1086195..1086665 - 471 WP_000136192.1 YlaC family protein -
GUU88_RS05330 1086781..1087332 - 552 WP_000102564.1 maltose O-acetyltransferase -
GUU88_RS05335 1087504..1087722 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GUU88_RS05340 1087748..1088122 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GUU88_RS05345 1088668..1091817 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GUU88_RS05350 1091840..1093033 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146820 WP_001291435.1 NZ_CP047594:c1087722-1087504 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146820 NZ_CP062792:3986071-3986289 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146820 WP_000344800.1 NZ_CP047594:c1088122-1087748 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146820 NZ_CP062792:3985670-3986044 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References