Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 550658..551234 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GUU88_RS02675 Protein ID WP_001297643.1
Coordinates 550658..550945 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GUU88_RS02680 Protein ID WP_000063156.1
Coordinates 550932..551234 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS02655 546911..548191 - 1281 WP_001037380.1 DUF445 domain-containing protein -
GUU88_RS02660 548232..549464 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
GUU88_RS02665 549610..549705 - 96 Protein_516 dienelactone hydrolase family protein -
GUU88_RS02670 549762..550459 + 698 Protein_517 IS1 family transposase -
GUU88_RS02675 550658..550945 + 288 WP_001297643.1 BrnT family toxin Toxin
GUU88_RS02680 550932..551234 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GUU88_RS02685 551268..552224 - 957 WP_001297640.1 GTPase -
GUU88_RS02690 552235..552438 - 204 WP_000467859.1 YbdD/YjiX family protein -
GUU88_RS02695 552488..554638 - 2151 WP_001736090.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T146817 WP_001297643.1 NZ_CP047594:550658-550945 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T146817 NZ_CP062792:1938194-1938296 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT146817 WP_000063156.1 NZ_CP047594:550932-551234 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT146817 NZ_CP062792:c1938303-1938158 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References