Detailed information of TA system TA146815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 315403..316001
• Replicon: chromosome
• Accession: NZ_CP047594
• Organism: Escherichia coli strain LD27-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GUU88_RS01485
• Protein ID: WP_053270478.1
• Coordinates: 315624..316001 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GUU88_RS01480
• Protein ID: WP_001603498.1
• Coordinates: 315403..315624 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU88_RS01460	310409..310957	+	549	WP_076486551.1	fimbrial protein YehD	-
GUU88_RS01465	311013..311693	+	681	WP_053270475.1	fimbrial assembly chaperone	-
GUU88_RS01470	311711..314197	+	2487	WP_089615834.1	fimbria/pilus outer membrane usher protein	-
GUU88_RS01475	314208..315218	+	1011	WP_089615833.1	fimbrial protein	-
GUU88_RS01480	315403..315624	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GUU88_RS01485	315624..316001	+	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GUU88_RS01490	316111..316326	-	216	Protein_284	transposase	-
GUU88_RS01495	316512..317774	-	1263	WP_089634553.1	integrase arm-type DNA-binding domain-containing protein	-
GUU88_RS01505	318154..318729	-	576	WP_001188520.1	transcriptional regulator	-
GUU88_RS01510	318766..320463	-	1698	WP_000068922.1	protein-disulfide reductase DsbD	-
GUU88_RS01515	320439..320777	-	339	WP_000883400.1	divalent cation tolerance protein CutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	291941..329655	37714
	-	flank	IS/Tn	-	-	316045..316326	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T146815 WP_053270478.1 NZ_CP047594:315624-316001 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T146815 NZ_CP062792:753412-753897 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAATGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT146815 WP_001603498.1 NZ_CP047594:315403-315624 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT146815 NZ_CP062792:753122-753415 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0