Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 272565..273062 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GUU88_RS01265 Protein ID WP_001173343.1
Coordinates 272889..273062 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GUU88_RS01260 Protein ID WP_000398619.1
Coordinates 272565..272861 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS01240 269296..270015 - 720 WP_000611288.1 two-component system response regulator DcuR -
GUU88_RS01245 270012..271643 - 1632 WP_001216477.1 sensor histidine kinase -
GUU88_RS01250 271824..272054 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
GUU88_RS01255 272066..272338 + 273 WP_000405651.1 N-acetyltransferase -
GUU88_RS01260 272565..272861 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GUU88_RS01265 272889..273062 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GUU88_RS01270 273181..274698 - 1518 WP_001295074.1 lysine--tRNA ligase -
GUU88_RS01275 274935..276392 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T146813 WP_001173343.1 NZ_CP047594:272889-273062 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T146813 NZ_CP062790:4588194-4588544 [Cytobacillus oceanisediminis]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGCGGCGTCCGTCCAGT
GCTTGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCAATCACAGCTCAGATTCAAAAAG
CGAAGCTGCCTACTCACGTTGAAATTGATGCGAAGCGTTATGGTTTTGAAAGGGATTCGGTCATCTTATTAGAACAGATT
CGTACAATTGATAAACAACGCTTAACCGATAAAATAACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCGT
GCAAATCAGCTTAGGCCTCATCGAATTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT146813 WP_000398619.1 NZ_CP047594:272565-272861 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT146813 NZ_CP062790:4587908-4588189 [Cytobacillus oceanisediminis]
GTGTCTGATTCCAGCACAACAACAGAGATAATGGTAAAGTTACCGCAGCATCTTTTAACCGAGTTAGACGGATTTGCAAA
ACAGGAAAACGTTAACCGGAGCGAATTCATTTATCAGGCAACCAAAATGTATTTGCGCGAACGCAAAAAGAGACAAATTC
GCGAGTCCATGAGACGAGGCTACATGGAGATGGCGAAAATAAATTTGACGATTGCATCTGAAGCATTTCAAGCGGAATAC
GAGGCAGAACACACAGTTGAACGTCTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References