Detailed information of TA system TA146812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1328831..1330494
• Replicon: chromosome
• Accession: NZ_CP047593
• Organism: Tichowtungia aerotolerans strain S-5007

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: GT409_RS05630
• Protein ID: WP_160627770.1
• Coordinates: 1329178..1330494 (+)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: GT409_RS05625
• Protein ID: WP_269844983.1
• Coordinates: 1328831..1329181 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT409_RS05605 (GT409_05605)	1324664..1325986	-	1323	WP_160627762.1	cell envelope integrity protein CreD	-
GT409_RS05610 (GT409_05610)	1326064..1326837	-	774	WP_160627764.1	HD domain-containing protein	-
GT409_RS05615 (GT409_05615)	1326840..1327379	-	540	WP_160627766.1	hypothetical protein	-
GT409_RS05620 (GT409_05620)	1327486..1328568	+	1083	WP_160627768.1	class II fructose-bisphosphate aldolase	-
GT409_RS05625 (GT409_05625)	1328831..1329181	+	351	WP_269844983.1	helix-turn-helix transcriptional regulator	Antitoxin
GT409_RS05630 (GT409_05630)	1329178..1330494	+	1317	WP_160627770.1	type II toxin-antitoxin system HipA family toxin	Toxin
GT409_RS05635 (GT409_05635)	1330529..1331872	-	1344	WP_160627771.1	sodium:alanine symporter family protein	-
GT409_RS05640 (GT409_05640)	1332112..1333068	+	957	WP_160627773.1	AEC family transporter	-
GT409_RS05645 (GT409_05645)	1333119..1333481	-	363	WP_160627775.1	hypothetical protein	-
GT409_RS05650 (GT409_05650)	1333645..1334865	-	1221	WP_160627387.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (174-410)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-67)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49247.89 Da
• Isoelectric Point: 6.2930

>T146812 WP_160627770.1 NZ_CP047593:1329178-1330494 [Tichowtungia aerotolerans]
MNPSVTADIILWGNTIGAVSWDAERELALFEYASDFLESGIELAPLTMPLRAQVYSFPELPRESFHGLPGMLADALPDRF
GNLLIDEWLVRTGRKQADFTPVERLCYIGRRGMGALEFRPAIRDRQSGSVPVNVAELVDLANTALAEKEMLATQINGKSE
DDLDAMRDILRVGTSAGGARAKAVIAWNDKTGDVRSGQVKAPPGFGYWLIKFDGVAGNRDKELNDPQGFGKIEYTYHRMA
LAAGIEMSECRLFEENGRCHFMTRRFDRTAEGNKIFMQSLCGIAHMDFNQAGAYGYEQAMDVALRLGLDAEALRQLFRRM
VFNLMARNQDDHTKNIAFLMNKSGDWSLAPAYDITYCYNPNGAWTRRHQMSVNGKHDHFERDDFMAVAKRFNLFKKAGVD
QVLEETADALRRWPEFAAQTGVPEQTVRQIAAHHRHIA

Nucleotide

• Length: 1317 bp

>T146812 NZ_CP062789:c3443436-3443056 [Janibacter indicus]
GTGATCGTCGTCGATGCCTCGGCGATGGTCGAGGCGCTCGTGGGGCGTGCGCCGGACCCGCGACTCCTCGACGCCCTTGC
CGGCGTGGTCGAGGCGCCGCAGCACCTGGACGTCGAGGTGCTGTCCGTCCTGCGGGGCCTGACGCTCGGGGCCAAGCTCC
CGGCGGCACGCGCCGAGGAGGCACTCGGGGCCTATCTCGACCTCGACATCACAAGACGGGACGTGGCTCCCTTCGCCCGG
AGGGTCTGGTCGCTGCGGCACCAGTACACGAGCTACGACGCGGCCTACCTGGCCCTGGCCGAGGGGCTCGACGCGCCGCT
CTACACGTGCGACCGCAAGCTGGCAGCCGGCGGTCACACGGCCGAGGTGGTCGTGATCTAG

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13156.32 Da
• Isoelectric Point: 11.0043

>AT146812 WP_269844983.1 NZ_CP047593:1328831-1329181 [Tichowtungia aerotolerans]
MMKTEAASNEAIRKELGKRLQSERLNQNITQAALAKKAGISRRTLVGAERGEPFTIDTMLSILRALDRLAQIDLFLPEPE
ISPVQLSKLKGKKRQRAGGKFTYPKPRNIPWVWNEE

Nucleotide

• Length: 351 bp

>AT146812 NZ_CP062789:c3443660-3443433 [Janibacter indicus]
ATGCCCACCCTGTACATCCGTGACGTCCCCGACGACGTGGCCGAGACGCTCAAGCAGCGTGCGGCGGCCGACGGGCAGTC
CCTGTCCGCTTACGTCGCCGGTGCGCTGCTTCGGCTCGCGGAACGCCCCACCAATGCCGAGGTCGTCGAGCGACTGCGGC
AGGTGGACCGGACATTGGCCCCGGCCTCCGAGGAGATCGTCTCGACGCTGCACGAGAGCCGCCGGTGA