Detailed information of TA system TA146806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 60482..61059
• Replicon: plasmid pSNYG35
• Accession: NZ_CP047590
• Organism: Proteus mirabilis strain SNYG35

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: GT733_RS18695
• Protein ID: WP_023159957.1
• Coordinates: 60727..61059 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GT733_RS18690
• Protein ID: WP_096864992.1
• Coordinates: 60482..60727 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT733_RS18650 (GT733_18740)	55504..55848	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
GT733_RS18655 (GT733_18745)	55841..56263	+	423	WP_172688766.1	hypothetical protein	-
GT733_RS18660 (GT733_18750)	56272..56664	+	393	WP_096864995.1	hypothetical protein	-
GT733_RS18665 (GT733_18755)	56944..57423	+	480	WP_048821772.1	Hcp family type VI secretion system effector	-
GT733_RS18670 (GT733_18760)	57432..58034	+	603	WP_241026953.1	hypothetical protein	-
GT733_RS18675 (GT733_18765)	58771..59139	+	369	WP_096864994.1	hypothetical protein	-
GT733_RS18680 (GT733_18770)	59237..59464	-	228	WP_096864993.1	plasmid partition protein ParG	-
GT733_RS18685 (GT733_18775)	59573..60196	-	624	WP_023159958.1	AAA family ATPase	-
GT733_RS18690 (GT733_18780)	60482..60727	+	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GT733_RS18695 (GT733_18785)	60727..61059	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
GT733_RS18700 (GT733_18790)	61090..61470	+	381	WP_096864991.1	hypothetical protein	-
GT733_RS18705 (GT733_18795)	61556..61699	-	144	WP_071547955.1	Hok/Gef family protein	-
GT733_RS18710 (GT733_18800)	62064..62861	-	798	WP_172688767.1	hypothetical protein	-
GT733_RS18715 (GT733_18805)	63463..64218	+	756	WP_159287658.1	site-specific integrase	-
GT733_RS18720 (GT733_18810)	64232..65050	+	819	WP_023159955.1	DUF4365 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / qacE / sul1 / mph(E) / msr(E) / aac(3)-IVa / aph(4)-Ia / sul2 / floR / dfrA14 / blaOXA-10 / ant(3'')-Ia / blaNDM-1	-	1..165923	165923
	-	inside	Integron	-	-	17..165921	165904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T146806 WP_023159957.1 NZ_CP047590:60727-61059 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T146806 NZ_CP062784:c29321-29055 [Escherichia coli O157:H7]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTGATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT146806 WP_096864992.1 NZ_CP047590:60482-60727 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT146806 NZ_CP062784:c29065-28823 [Escherichia coli O157:H7]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75