Detailed information of TA system TA146804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3708356..3709005
• Replicon: chromosome
• Accession: NZ_CP047589
• Organism: Proteus mirabilis strain SNYG35

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: GT733_RS17005
• Protein ID: WP_012368534.1
• Coordinates: 3708356..3708775 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: GT733_RS17010
• Protein ID: WP_012368535.1
• Coordinates: 3708772..3709005 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT733_RS16975 (GT733_17045)	3703635..3704513	-	879	WP_017628653.1	23S rRNA pseudouridine(2604) synthase RluF	-
GT733_RS16980 (GT733_17050)	3704689..3705603	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
GT733_RS16985 (GT733_17055)	3705627..3706064	-	438	WP_004246810.1	D-aminoacyl-tRNA deacylase	-
GT733_RS16990 (GT733_17060)	3706145..3706762	-	618	WP_004246809.1	glucose-1-phosphatase	-
GT733_RS16995 (GT733_17070)	3707414..3707830	-	417	WP_046334846.1	hypothetical protein	-
GT733_RS17000 (GT733_17075)	3707808..3708131	-	324	WP_104731870.1	hypothetical protein	-
GT733_RS17005 (GT733_17080)	3708356..3708775	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
GT733_RS17010 (GT733_17085)	3708772..3709005	-	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GT733_RS17015	3709272..3709424	+	153	WP_172764486.1	hypothetical protein	-
GT733_RS17020 (GT733_17090)	3710384..3711592	+	1209	WP_060555750.1	DUF262 domain-containing protein	-
GT733_RS17025 (GT733_17095)	3711809..3712030	-	222	WP_060555751.1	hypothetical protein	-
GT733_RS17030 (GT733_17100)	3712078..3712365	+	288	WP_104731871.1	transposase	-
GT733_RS17035 (GT733_17105)	3712815..3713294	+	480	WP_060555753.1	Hcp family type VI secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T146804 WP_012368534.1 NZ_CP047589:c3708775-3708356 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T146804 NZ_CP062783:71584-71742 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT146804 WP_012368535.1 NZ_CP047589:c3709005-3708772 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT146804 NZ_CP062783:71321-71519 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0